Erschienen: 19.10.2018 Abbildung von Yan Shan | Engineering a Robust DNA Circuit for the Direct Detection of Biomolecular Interactions | 1st ed. 2018 | 2018

Yan Shan

Engineering a Robust DNA Circuit for the Direct Detection of Biomolecular Interactions

1st ed. 2018 2018. Buch. xlv, 188 S. 87 s/w-Abbildungen, 30 Farbabbildungen, 117 Farbtabellen, Bibliographien. Hardcover

Springer. ISBN 9789811321870

Format (B x L): 15,5 x 23,5 cm

Gewicht: 520 g

In englischer Sprache

Das Werk ist Teil der Reihe: Springer Theses

Produktbeschreibung

This book provides essential insights into designing a localized DNA circuit to promote the rate of desired hybridization reactions over undesired leak reactions in the bulk solution. The area of dynamic DNA nanotechnology, or DNA circuits, holds great promise as a highly programmable toolbox that can be used in various applications, including molecular computing and biomolecular detection. However, a key bottleneck is the recurring issue of circuit leakage. The assembly of the localized circuit is dynamically driven by the recognition of biomolecules – a different approach from most methods, which are based on a static DNA origami assembly. The design guidelines for individual reaction modules presented here, which focus on minimizing circuit leakage, are established through NUPACK simulation and tested experimentally – which will be useful for researchers interested in adapting the concepts for other contexts. In the closing section, the design concepts are successfully applied to the biomolecular sensing of a broad range of targets including the single nucleotide mutations, proteins, and cell surface receptors.

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