Erschienen: 01.09.1979 Abbildung von Melchers / Potter / Warner | Lymphocyte Hybridomas | 1979

Melchers / Potter / Warner

Lymphocyte Hybridomas

Second Workshop on “Functional Properties of Tumors of T and B Lymphoyctes”

lieferbar ca. 10 Tage als Sonderdruck ohne Rückgaberecht

117,69 €

inkl. Mwst.

1979. Buch. xxiv, 248 S. 25 s/w-Abbildungen, Bibliographien. Softcover

Springer. ISBN 978-3-540-09670-2

Format (B x L): 15,6 x 24,4 cm

Gewicht: 520 g

In englischer Sprache

Produktbeschreibung

F. Melchers, M. Potter, NL. Warner HISTORICAL Prior to 1964 many workers had experienced considerable difficulty in establishing continuous lines of murine plasmacytoma in culture. Pettingill and Sorenson in 1966 grew X5563 after a painstaking period of adaptation. During a visit to the Salk Instiute in 1965 Leo Sachs began experiments with K. Horibata,E. S. Lennox and M. Cohn that ulti­ mateley convinced most workers that murine plasmacytomas could be grown in vitro with ease. They adapted MOPC-21 * {called P3 at the Salk Institute) to in vitro culture and showed the cells produced a large amount of the IgG1 myeloma protein. Horibata, Lennox and Sachs did not report the important finding though a manuscript was written and accepted. The reason for the failure to publish the paper was that the original line was not recovered from the freezer and knowing there would be many requests for it the paper was withdrawn. As it later turned out, the failure to recover the line may have been triv­ ial, for Horibata and Harris (1970) soon re-established a continuous line of P3 now named P3K. This line was distributed to other workers including Cesar Milstein in Cambridge. Soon after the success with P3, Scharff and colleagues at Albert Einstein established MPC-11*in cul­ ture. Up to now continuous growth of human myeloma cells in vitro has remained a formidable problem.

Gesamtwerk

Die 8. Auflage ist wieder auf sechs Bände angelegt. Darin finden sich übersichtlich und in systematischer Gliederung Vertragsmuster aus der Feder erfahrener Experten. Jedem dieser Muster folgen Anmerkungen, mit denen der dem Vertragsentwurf zu Grunde liegende Sachverhalt und die Gründe für die Wahl des spezifischen Formulars erläutert werden.

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